This stain is a 1% aqueous solution, and its especially useful for microscope slide preparation. The methylene blue value is a function of the amount and characteristics of clay minerals present in the test specimen. Methylene blue 1% aqueous solution breckland scientific. Methylene blue active substances mbas assay for nonionic surfactants in environmental samples. Sigmaaldrich offers a number of methylene blue products. Safety data sheet methylene blue prolab diagnostics. Basic blue 9 trihydrate recommended use laboratory chemicals. Methylene blue reduction test reflects the bacterial load in milk and it is indicated by time taken for methylene blue dye in milk to change in color from blue to white. Safety data sheet according to federal register vol. Place two 589wh filter paper on top of the plastic ring on top of the samples. Methylene blue stains to negatively charged cell components like nucleic acids.
A rapid determination of sodium dodecyl sulfate with. If released to the atmosphere, methylene blue will exist as both vapor and particulate in the ambient atmosphere. Methylene blue safety data sheet according to federal register vol. Methylene blue cas 61734 scbt santa cruz biotechnology. According to 19072006ec reach, 4532010eu, 2015830eu. Product identification name methylene chloride synonyms dichloromethane. Methylene blue is a compound that occurs as crystals or crystalline powder, having a bronzelike luster. Chemical product and company identification product name. Methylene blue stock solution was diluted 100fold with 0. Pdf diffuse reflectance dr spectra in the visible vis and nearinfrared nir region of methylene blue mb adsorbed on clay samples of different. According to regulation ec no 19072006, annex ii, as amended. Put other soil sample on top, keeping the filter paper in between in intimate contact with soil samples.
As an experimental pharmaceutical drug, the international nonproprietary name i nn of methylene blue is methylthioninium chloride. Collect 1 ml of sample and place into 15 ml conical tube 2. Vaporphase methylene blue is degraded in the atmosphere by reaction with photochemically produced hydroxyl radicals with an estimated halflife of about 1. Methylene blue is used as a bacteriologic stain or dye and indicator. It has previously been used for cyanide poisoning and urinary tract infections, but this use is no longer recommended. Put the lid on and tape it tight to prevent moisture lost. The result is reported as a methylene blue value in units of mg of methylene blue adsorbed per g of fine aggregate or mineral filler. Methylene blue active substances mbas assay for nonionic. According to regulation ec no 1907 2006, annex ii, as amended. Product identifier product name methylene blue product number.
It is a photoactive dye, which is a member of phenothiazine family. Other hazards which do not result in classification other hazards not contributing to the classification. Chemtrec 8004249300 outside usa 7035273887 1216 6th ave n kent wa 98032 phone. It is soluble in water, chloroform and partially soluble in alcohol. Add 1ml of methylene blue dye to the sample and close the. Skin contact wash off immediately with plenty of water for at least 15 minutes. This modified methylene blue procedure allows the detection and quantitation. This material is provided for educational purposes only and is not intended for medical advice, diagnosis or treatment. Methylene chloride revision date 28nov2019 eye contact rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Record reduction time in whole hours between last inversion and decolorization. Pipette 10 ml of deionized water into the erlenmeyer flask. Safety data sheet creation date 22feb2010 revision date 18jan2018 revision number 3 1. It is often used in combination with ziehlneelsen carbol fuchsin solution and hydrochloric acid in. As a medication, it is mainly used to treat methemoglobinemia.
For example, if the sample were still blue after l 5 hours but was decolorized white at the 2. Safety data sheet of eosin methylene blue agar e05102 version 1. Decolorization is considered complete when fourfifths of the color has disappeared. Central drug house p ltd 728 vardaan house new delhi2 india.
Methylene blue, also known as methylthioninium chloride, is a medication and dye. Methylene blue aqueous staining material safety data. In addition, methylene blue was found to significantly inhibit sperm motility in vitro in samples prepared from human semen. Methylene blue is a dye that was first developed to stain and inactivate certain microbes. To accomplish work, the published version of the document should be viewed online. Total sulfide, methylene blue method page 3 of 10 printouts of this document may be out of date and should be considered uncontrolled. Ph eur find msds or sds, a coa, data sheets and more information. Add 3 ml of chloroform in fume hood to each sample 4. Uses advised against food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet. Safety data sheet for methylene blue solution, product number. Identification of the material and supplier product name.
Identification of the substancemixture and of the companyundertaking 1. Pdf an assay evaluation of the methylene blue method for the. Source of sample, andrews paper and chemical company, inc. A rapid determination of sodium dodecyl sulfate with methylene blue. Data sources include ibm watson micromedex updated 4 may 2020, cerner multum updated 4 may 2020, wolters kluwer updated. Safety data sheet methylene blue pro lab diagnostics inc. Specifically, it is used to treat methemoglobin levels that are greater than 30% or in which there are symptoms despite oxygen therapy. Methylene blue was first prepared in 1876 by german chemist heinrich caro 1 8341910. Methylene blue kit instruction manual 209680 revision e, april 20 11 4.
Wear appropriate personal protective equipmentsee section 8. It was also one of the first chemotherapeutic medications tested in humans, where it was used to treat. Identification of the substancemixture and of the company. Based on the analysis of negative samples, a urine specimen with an anionic. Methylene chloride please ensure that this msds is given to, and explained to people using this product. Use the syringe to accurately measure 2 ml of freshly agitated drilling fluid. Insert the jar into a well insulated container for suction equilibrium. Certificate of analysis safety data sheet sds sds canada english sds canada french. The methylene green sds detection assay utilizes the partitioning of.
Methylene blue, loeffler safety data sheet according to federal register vol. It is a traditional method which is followed throughout the globe. Methylene blue has been found to cause an elevation in follicle stimulating hormone and estradiol in fallopian tube secretions, and uterine and peritoneal fluids. Safety data sheet methylene blue according to regulation ec no 19072006, annex ii, as amended. According to 19072006 ec reach, 4532010eu, 2015830eu. Samples or standard solutions equivalent to 010,ug of sids up to l ml in volume were placed in optically suitable small test tubes of inner diameter 1. Methylene blue injection, usp 1% 1 of 7 safety data sheet 1. Methylene blue msds 17012019 page 1 material safety data sheet methylene blue. Methylene blue stain is a solution used to highlight certain cell parts of animal, bacteria, and blood tissue specimens. Material safety data sheet methylene blue msds section 1.
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